4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:糖苷内/外切酶,去糖基化酶,唾液酸产品,多糖产品纯化试剂盒,多糖标记试剂盒,HPLC柱及其缓冲液
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Primer_Probe > QA Bio/2-AB标记试剂盒/LT-KAB-VP24
商品详细QA Bio/2-AB标记试剂盒/LT-KAB-VP24
QA Bio/2-AB标记试剂盒/LT-KAB-VP24
QA Bio/2-AB标记试剂盒/LT-KAB-VP24
商品编号: LT-KAB-VP24
品牌: qabio
市场价: ¥6722.82
美元价: 5171.40
产地: 美国(厂家直采)
公司:
产品分类: 引物与探针
公司分类: Primer_Probe
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

The2-ABlabelingkitcontainstwosetsofthefollowingreagents(15samplesperkit)suppliedinglassampoulessealedunderpurenitrogen:  2-Aminobenzamide(2-ABdye)  Dimethylsulfoxide(DMSO)  Aceticacid  Sodiumcyanoborohydrideor2-picolineborane

TrADItional2-AAand2-ABlabellingkitsusesodiumcyanoborohydrideasareducingagentduringglycanlabeling.Thisreagentistoxicsoafumecupboardshouldbeusedduringhandling.ToconformwithemerginghealthandsafetyregulationswearenowreplacingthesewithournewVPglycankitsthatusepicolineboranewhichisasignificantlysaferreductant.

NumberofSamplesOne2-ABlabelingkitcontainsreagentstolabelupto30separateanalyticalsamplesperkit

Dyepurity>99%byHPLC

Molecularweight137

LamBDa-ex320nm

Lambda-em420nm

AmountofSampleFrom25pmolupto25nmolglycanspersample./p>

SuitableSamplesAnypurifiedglycanswithfreereducingterminicanbelabeled.

StructuralIntegrityNodetectable(<2molepercent)lossofsialicacid,fucose,sulfate,orphosphate.

LabelingEfficiencyTypically>85%(dependentonsample).

LabelingSelectivityEssentiallystoichiometriclabeling.

Protocol1. PurifytheglycansLudgerCleanEB10cartidges(LC-EB10-A6)havebeendesignedforpurificationofglycansfromproteins,salts,anddetergents.

2.TransfersampletoreactionvialTheamountofsampleshouldbeintherange100picomoles–50nanomolesforaglycanpoolobtainedfromatypicalglycoprotein.Withasinglepureglycanaslittleas5picomolescanbelabeledanddetectedinsubsequentHPLCanalysis.SuitablereactionvialsincludesmallpolypropylenemicrocentrifugetubesandtubesforPCRwork.

3. DrythesamplesIdeally,samplesshouldbedriedusingacentrifugalevaporator.IfthisisnotpossIBLethenfreezedrying(lyophilization)canbeusedwithcaution(inparticular,ensurethatthesampledriestoasmall,compactmassattheverybottomofthevial).Donotsubjectsamplestohightemperatures(>28°C)orextremesofpHastheseconditionswillresultinacidcatalysedlossofsialicacids(hightemperatures,lowpH)orepimerizationoftheglycanreducingterminus(athighpH).

4.PrepareaDMSO-aceticacidmixtureAdd150μLglacialaceticacidtothevialofDMSOandmixbyPipetteaction.

5.AddthedyeAdd100μLoftheDMSO-aceticacidmixturetoavialofthe2-AB(2-AminobenzamideAcid)dyeandmixuntilthedyeisdissolved.

6.AddthereductantAddthedissolveddyetoavialofsodiumcyanoborohydrideorpicolineboraneandmixbypipetteactionuntilthereductantiscompletelydissolvedtomakethefinallabelingreagent.

7.AddlabelingreagenttosamplesAdd5μLoflabelingreagenttoeachdriedglycansample,capthemicrotube,mixthoroughly.

8.IncubatePlacethereactionvialsinaheatingblock,sandtray,ordryovensetat65°Candincubatefor3hours.Inmostcases,theincubationtimecanbeshortenedto2hoursorextendedupto4hourswithoutsignificantlychangingtheoutcomeofthelabelingreaction.

9.CentrifugeandcoolAftertheincubationperiodremovethesamples,centrifugethemicrotubesbriefly,thenallowthemtocoolcompletelytoroomtemperature.

10.SampleCleanupPost-labelingsamplecleanupisrecommendedtoremoveexcessdyeandotherlabelingreagents.CleanupcanbeachievedusingLudgerCleanT1cartridges(LC-T1-A6)orScartridges(LC-S-A6)

2-ABLabelingReferences

AnumulaKR,DhumeST.Highresolutionandhighsensitivitymethodsforoligosaccharidemappingandcharacterizationbynormalphasehighperformanceliquidchromatographyfollowingderivatizationwithhighlyfluorescentanthranilicacid.GlycoBIOLOGy8685-694(1998)

BiggeJC,PatelTP,BruceJA,GouldingPN,CharlesSM,ParekhRB.Nonselectiveandefficientfluorescentlabelingofglycansusing2-aminobenzamideandanthranilicacid.AnalBiochem230:229-238(1995)

Guile,G.R.;Rudd,P.M.;Wing,D.R.;Prime,S.B.;Dwek,R.A.Arapidandhigh-resolutionhigh-performanceliquidchromatographicmethodforseparatingglycanmixturesandanalyzingoligosaccharideprofilesAnalyticalBiochemistry240:210-226(1996)

Hardy,M.R.‘Glycanlabelingwiththefluorophores2-aminobenzamideandanthranilicacid’in‘TechniquesinGlycobiology’,editedbyTownsend,R.RandHotchkiss,A.T..MarcelDekkerInc,NewYork(1997)

品牌介绍
QA-bio公司是一家致力于提供糖基化研究工具的专业公司,所提供的糖基化研究产品涵盖糖苷内/外切酶,去糖基化酶,唾液酸产品,多糖产品纯化试剂盒,多糖标记试剂盒,HPLC柱及其缓冲液,同时公司还提供多糖分析服务。此外,公司还提供部分分子生物学产品。