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4000-520-616
当前位置: 首页 > 产品中心 > saccharide > QA-Bio/IgG-聚糖库/CPM-C13-IgG-01
商品详细QA-Bio/IgG-聚糖库/CPM-C13-IgG-01
QA-Bio/IgG-聚糖库/CPM-C13-IgG-01
QA-Bio/IgG-聚糖库/CPM-C13-IgG-01
商品编号: CPM-C13-IGG-01
品牌: qabio
市场价: ¥2598.96
美元价: 1999.20
产地: 美国(厂家直采)
公司:
产品分类: 糖类
公司分类: saccharide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

OurIgGglycanlibraryisamixtureofnonfucosylatedandfucosylated,bi-antennaryglycanstandardswithvariablesialylationreleasedfromhumanIgGantibodyglycoprotein.Theyareavailableunlabeledandlabeledwithavarietyoftags.

WealsosupplyanIgGglycoproteinstandardforuseduringglycanreleaseandlabeling.

“Glycosylationcansignificantlyaffecttheinvivosafetyandefficacyprofilesoftherapeuticrecombinantmonoclonalantibodies(rMAbs).Inparticular,glycanscanhaveamarkedinfluenceonIgGFceffectorfunctionsandchangesinantibodyglycosylationareamajorcauseofbatch-to-batchvariABIlityduringproduction.Forthesereasons,itisessentialtomeasureandcontrolantibodyglycosylationaccuratelyandreliably.”ThisintroductionwascopiedfromDr.DarylFernandes’articleDemonstratingComparabilityofAntibodyGlycosylationduringBiomanufacturing

Sources: TheIgGglycanlibraryintheseproductsarereleasedfromanIgGstandardthatispurifiedfromhumanserum.IgGexistsinavarietyofglycoformscontainingbi-antennaryoligosaccharideswithvariablesialylation.

Form: Dry.Driedbycentrifugalevaporationfromanaqueoussolution.

Amountsupplied
    CLIBN-IGG-01      25µgs–unlabeled
    CAB-IGG-01          200pmol–2-ABlabeled
    CAPTS-IGG-01     10pmol–APTSlabeled
    CPROC-IGG-02    50pmol–procainamidelabeled
    CPM-IGG-01         20MSruns–permethylated
    CPM-C13-IGG-01  20MSruns–permethylatedwithheavy(13C)MeI

PermethylatedIgGglycanstandardsforMSanalysis
Massspectrometry(MS)isausefultoolindeterminingthemolecularweightsbutthesensitivitycanbelowespeciallywheretherearemanydifferentstructures.Carbohydratesoftencontaincarboxy,amino,sulphate,andphosphategroups.Thenatureandthepositionofthesegroupsontheresidue,aswellasthepositionofthisresiduewithintheglycanmaybedifficulttodetermine.Permethylationofglycansconvertshydrogengroupstomethylgroupswhichrenderstheglycanshydrophobic;theconversionstabilisessialicacidsandcanincreasesignalintensityinmassmeasurements.

WenowofferaLudgerTagapermethylatedIgGglycanlibrarytouseasasystemsuitabilitystandardduringMSanalysis.WealsoofferaC13versionwhichcanbeusedasaninternalstandardinthesameMALDIchipspotasyourC12-labelledpermethylatedglycans.

IgG Permthylation data
MassspectrumofpermethylatedIgGN-GlycansstandardreleasedfromHumanIgGantibodybyN-Modehydrazinolysis.AnalysisperformedonBrukerAutoflexMALDI-Ion

Purity: >90%pureasassessedbyacombinationof1H-NMRandHPLC,andCE

Storage: -20˚Cbothbeforeandafterdissolution.Theproductsarestableforatleast5yearsassupplied.
ViewCertificateofStabilty

Shipping: Theproductcanbeshippedatambientwhendry.Afterdissolution,shipondryice.

Handling: Allowtheunopenedvialtoreachambienttemperatureandtapunopenedonasolidsurfacetoensurethatmostofthelyophilizedmaterialisatthebottomofthevial.Gentlyremovethecap,addthedesiredvolumeofreconstitutionmedium,re-capandmixthoroughlytobringalltheoligosaccharideintosolution.Formaximalrecoveryofoligosaccharide,ensurethatthecapliningisalsorinsedandcentrifugethereconstitutedvialbrieflybeforeuse.Ensurethatanyglass,plasticwareorsolventsusedarefreeofglycosidasesandenvironmentalcarbohydrates.MinimiseexposuretoelevatedtemperaturesorextremesofpH.HightemperaturesandlowpHwillcausedesialylation.HighpHwillcauseepimerisationofthereducingterminusGlcNAc.

Safety: Thisproductisnon-hazardousandhasbeenpurifiedfromnaturalsourcescertifiedtobefreeofallhazardousmaterialincludingpathogenicBIOLOGicalagents.
DownloadNon-hazardousMSDS

References

BeyerB,SchusterM,JungbauerA,LinggN.MicroheterogeneityofRecombinantAntibodies:AnalyticsandFunctionalImpact.BiotechnolJ.2018Jan;13(1).doi:10.1002/biot.201700476.Epub2017Sep25.Review.

ShaS,AgarabiC,BrorsonK,LeeDY,YoonS.N-GlycosylationDesignandControlofTherapeuticMonoclonalAntibodies.TrendsBiotechnol.2016Oct;34(10):835-846.doi:10.1016/j.tibtech.2016.02.013.Epub2016Mar22.Review

LiuL.AntibodyglycosylationanditsimpactonthepharmacokineticsandpharmacodynamicsofmonoclonalantibodiesandFc-fusionproteins.JPharmSci.2015Jun;104(6):1866-1884.doi:10.1002/jps.24444.Epub2015Apr14.

JefferisR.Recombinantantibodytherapeutics:theimpactofglycosylationonmechanismsofaction.TrendsPharmacolSci.2009Jul;30(7):356-62.doi:10.1016/j.tips.2009.04.007.Epub2009Jun22.Review.

JefferisR.Glycosylationofrecombinantantibodytherapeutics.BiotechnolProg21:pp11-16,2005

FernandesD.DemonstratingComparabilityofAntibodyGlycosylationduringBiomanufacturing.EuropeanBiopharmaceuticalReview.Summer2005.pp106-110

品牌介绍
QA-bio公司是一家致力于提供糖基化研究工具的专业公司,所提供的糖基化研究产品涵盖糖苷内/外切酶,去糖基化酶,唾液酸产品,多糖产品纯化试剂盒,多糖标记试剂盒,HPLC柱及其缓冲液,同时公司还提供多糖分析服务。此外,公司还提供部分分子生物学产品。