Procainamidelabelingpermitsglycanidentificationbyeithermassspectrometryor(U)HPLC.Becauseofitsimprovedionisationefficiencycomparedto2ABlabelingitcanpermitidentificationofminorglycans(<1% relative="" peak="" area)="" by="">

Ludger’sprocainamidelabelingsystemissuitableforN-glycans,O-glycans,GSL-glycans,heparinoranysugarwithareducingterminusandusesthesamereductiveaminationlabelingmethodthathasbeenusedfor2AB&2AA.

Thekitcontainsreagentsfortheconjugationofdyetothefreereducingendoftheglycanbyareductiveaminationreactionwithessentiallystoichiometriclabeling.Anypurifiedglycanswithfreereducingterminicanbelabeled.Upto12separateanalyticalsamplespersetoflabelingreagents(24samplesintotalforthekit).From25pmolupto25nmolglycanscanbelabeledpersample.

ThisLudgerTagkit,alongwithapost-labellingcleanupplateforthesamples(catalog#LC-PROC-96),hasbeenvalidatedinhouseatLudger.TypicalCVsfortriplicateanalyseswere5%.

StructuralIntegrityNodetectable(<2molepercent)lossofsialicacid,fucose,sulfate,orphosphate.

StorageStoreatroomtemperatureinthedark.Protectfromsourcesofheat,light,andmoisture.Thereagentsarestableforatleasttwoyearsassupplied.

ShippingTheproductcanbeshippedatambienttemperature.

Procainamidereferences

CrescioliS,ChiaruttiniG,MeleS,IlievaKM,PellizzariG,SpencerDIR,GardnerRA,LacyKE,SpicerJF,TuttANJ,WagnerGK,KaragiannisSNEngineeringandstableproductionofrecombinantIgEforcancerimmunotherapyandAllergoOncologyJournalofAllergyandClinicalImmunologyJan31.pii:S0091-6749(18)30081-2(2018)

KozakRP,TortosaCB,FernandesDL,SpencerDIComparisonofprocainamideand2-aminobenzamidelabelingforprofilingandidentificationofglycansbyliquidchromatographywithfluorescencedetectioncoupledtoelectrosprayionization-massspectrometryAnalBiochem.Oct1;486:38-40(2015)

ThomsonRI,GardnerRA,StrohfeldtK,FernandesDL,StaffordGP,SpencerDIR,OsbornHMIAnalysisofThreeEpoetinAlphaProductsbyLCandLC-MSIndicatesDifferencesinGlycosylationCriticalQualityAttributes,IncludingSialicAcidAnalyticalChemistryContentJun20;89(12):6455-6462(2017)