Procainamidelabelingpermitsglycanidentificationbyeithermassspectrometryor(U)HPLC.Becauseofitsimprovedionisationefficiencycomparedto2ABlabelingitcanpermitidentificationofminorglycans(<1% relative="" peak="" area)="" by="">1%>
Ludger’sprocainamidelabelingsystemissuitableforN-glycans,O-glycans,GSL-glycans,heparinoranysugarwithareducingterminusandusesthesamereductiveaminationlabelingmethodthathasbeenusedfor2AB&2AA.
Thekitcontainsreagentsfortheconjugationofdyetothefreereducingendoftheglycanbyareductiveaminationreactionwithessentiallystoichiometriclabeling.Anypurifiedglycanswithfreereducingterminicanbelabeled.Upto12separateanalyticalsamplespersetoflabelingreagents(24samplesintotalforthekit).From25pmolupto25nmolglycanscanbelabeledpersample.
ThisLudgerTagkit,alongwithapost-labellingcleanupplateforthesamples(catalog#LC-PROC-96),hasbeenvalidatedinhouseatLudger.TypicalCVsfortriplicateanalyseswere5%.
StructuralIntegrityNodetectable(<2molepercent)lossofsialicacid,fucose,sulfate,orphosphate.
StorageStoreatroomtemperatureinthedark.Protectfromsourcesofheat,light,andmoisture.Thereagentsarestableforatleasttwoyearsassupplied.
ShippingTheproductcanbeshippedatambienttemperature.
Procainamidereferences
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KozakRP,TortosaCB,FernandesDL,SpencerDIComparisonofprocainamideand2-aminobenzamidelabelingforprofilingandidentificationofglycansbyliquidchromatographywithfluorescencedetectioncoupledtoelectrosprayionization-massspectrometryAnalBiochem.Oct1;486:38-40(2015)
ThomsonRI,GardnerRA,StrohfeldtK,FernandesDL,StaffordGP,SpencerDIR,OsbornHMIAnalysisofThreeEpoetinAlphaProductsbyLCandLC-MSIndicatesDifferencesinGlycosylationCriticalQualityAttributes,IncludingSialicAcidAnalyticalChemistryContentJun20;89(12):6455-6462(2017)