4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:糖苷内/外切酶,去糖基化酶,唾液酸产品,多糖产品纯化试剂盒,多糖标记试剂盒,HPLC柱及其缓冲液
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Primer_Probe > QA生物/二甲基化试剂盒/LT-PERMET-VP24
商品详细QA生物/二甲基化试剂盒/LT-PERMET-VP24
QA生物/二甲基化试剂盒/LT-PERMET-VP24
QA生物/二甲基化试剂盒/LT-PERMET-VP24
商品编号: LT-PERMET-VP24
品牌: qabio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 引物与探针
公司分类: Primer_Probe
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

KitContents


96wellPermethylationplate1Plate
Dimethylsulfoxide(DMSO)40.0ml
Dichloromethane(DCM)60.0ml
96wellBalancePlate1Plate
96wellPlateLid1Lid/Mat

Thepermethylationplate(LC-PERMET-96)isscalableandcanbeusedforanynumberofsamplesbetween1and96.Storetheunusedkitreagentsandtheunusedplatewellsintherecommendedstorageconditionstoenabletheiruseforfurthersamplepreparationatalaterdate.

Note:Duetoshippingrestrictionsofmethyliodide/iodomethane,weareunabletoprovidethiscomponentwiththekit.Werecommendpurchasingiodomethanewithpurity≥99.0%(GC)fromyourlocalchemicalprovider.IodomethaneSynonym:methyliodideCASNumber:74-88-4

 

TimelinefortheProcedure

Step1:Permethylationofsamples
a.PreparationofglycansAsrequired
b.AdditionofDMSOandsampletoplate15minutes
c.Incubation15minutes
d.AdditionofMeItopermethylationplate15minutes
e.Incubation60minutes

Step2:Extractionofpermethylatedsamplesandstorage
a.Extractionofsamples3-4hours
b.Dryingpermethylatedsamples10-30minutes
c.Additionof70%MeOHforstorage10-20minutes

 

PermethylationReferences

A.Dell;A.J.Reaseon;Kay-HooiKhoo;M.Panico;A.McDowellandH.R.MorrisMassSpectrometryofCarbohydratecontainingBiopolymerMethodsinEnzymology230:108-132(1994)

I.Ciucanu;C.E.CostelloEliminationofOxidativeDegradationduringtheper-O-MethylationofCarbohydratesJ.Am.Chem.Soc.125:16213-16219(2003)

I.CiucanuPer-O-MethylationreactionofstructuralanalysisofcarbohydratesbymassspectrometryAnalyticaChimicaActa576:147-155(2006)

I.Ciucanu;R.CapritaPer-O-MethylationofneutralcarbohydratesdirectlyfromaqueoussamplesforgaschromatographyandmassspectrometryanalysisAnalyticaChimicaActa585:81-85(2007)

Shubhakar,A.,Kozak,R.P.,Reiding,K.R.,Royle,L.,Spencer,D.I.R.,Fernandes,D.L.,Wuhrer,M.AutomatedHigh-ThroughputPermethylationforGlycosylationAnalysisofBIOLOGicsusingMALDI-TOF-MSAnalyticalChemistry.acs.analchem.6b01639(2016).

品牌介绍
QA-bio公司是一家致力于提供糖基化研究工具的专业公司,所提供的糖基化研究产品涵盖糖苷内/外切酶,去糖基化酶,唾液酸产品,多糖产品纯化试剂盒,多糖标记试剂盒,HPLC柱及其缓冲液,同时公司还提供多糖分析服务。此外,公司还提供部分分子生物学产品。